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目的:分析KCl是否会引起Hepa1-6细胞胞内钙离子浓度的升高,并解释相关机理。方法:KCl的浓度和负载时间可能会引起细胞内钙离子浓度的不同变化。采用比率荧光成像系统检测Hepa1-6细胞的胞内钙离子浓度。结果:(1)在即时检测组中,与对照组相比,10、30、50mmol/L的KCl分别引起胞内钙离子浓度的显著升高(p<0.05),但各组别之间无显著差异。(2)在0.5h延迟检测组中,与对照组相比,10、30、50mmol/L的KCl分别引起胞内钙离子浓度的显著升高(p<0.05)并且各组别之间无显著差异。此外,即时检测组与延迟检测组的结果没有显著差异。结论:10mmol/L的KCl足以引起Hepa1-6细胞胞内钙离子浓度即时的显著上升。无论在即时检测组还是0.5h延迟检测组,各组别之间无显著差异。这表明当胞内的钙离子浓度上升到一定值,KCl对于胞内钙离子浓度的影响不会随其浓度的变化而发生改变。
OBJECTIVE: To analyze whether KCl can induce the increase of intracellular calcium concentration in Hepa1-6 cells and explain the related mechanism. Methods: KCl concentration and loading time may cause different changes in intracellular calcium concentration. Intracellular calcium concentration of Hepa1-6 cells was measured by a ratio fluorescence imaging system. Results: (1) Compared with the control group, KCl at 10, 30, 50 mmol / L caused a significant increase in intracellular calcium concentration (p <0.05) in the immediate testing group, but no Significant differences. (2) Compared with the control group, KCl at 10, 30 and 50 mmol / L induced a significant increase in intracellular calcium concentration (p <0.05) and no significant difference among the groups difference. In addition, there was no significant difference between the results of the instant test group and the delayed test group. Conclusion: 10 mmol / L KCl is enough to induce an immediate and significant increase of intracellular calcium concentration in Hepa1-6 cells. There was no significant difference between the groups in either the immediate-test group or the 0.5-h delayed test group. This shows that when the intracellular calcium concentration rises to a certain value, the influence of KCl on the intracellular calcium concentration does not change with its concentration.