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研制了纳喷雾离子化技术与正交飞行时间质谱的联用接口 ,在电场力的作用下 ,样品流速约为 30nL/min ,4 0nmol/L细胞色素c溶液的单张图谱样品消耗量为 0 0 2amol、检测限约为 0 8～ 1 2zmol.由于纳喷雾离子化技术的离子化机理与常规离子化方法有所不同 ,由母液滴裂解成能被直接检测的液滴的步骤少 ,因此能增加检测的灵敏度 ,特别适合于分析多糖类分子 .通过分析SBE β CD获得了较满意的结果 .另外还分别对agiotensinⅠ进行了源内CID实验和对细胞色素c的胰酶酶解图谱进行分析 ,对所有酶解的多肽混合物离子作了归属 .证明纳喷雾技术是一种高灵敏度 ,且适合于分析复杂基质环境及微量样品的技术 .
Under the action of electric field force, the sample flow rate is about 30nL / min, and the consumption of the single pattern of 40nmol / L cytochrome c solution is 0 0 2amol, the detection limit is about 0 8 ~ 1 2zmol.Because the ionization mechanism of nanospray ionization is different from the conventional ionization method, there are few steps for the liquid droplet to be directly detected by mother liquid drop, Increase the sensitivity of the detection and is particularly suitable for the analysis of polysaccharide molecules.According to the analysis of SBEβCD obtained more satisfactory results.In addition, respectively, the agiotensin Ⅰ in-source CID experiments and trypsin digestion of cytochrome c mapping analysis, All enzymatic peptide mixture ions were assigned, demonstrating that the nanospray technique is a highly sensitive technique suitable for the analysis of complex matrix environments and trace samples.