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使用正交试验结合单柱分离,对甘草黄酮原料进行前处理。正交试验优化得到的最佳萃取条件为:原料质量浓度选择30g/L,采用乙醇做提取溶剂,超声提取60min,提取温度20℃得到提取液浓度为10g/L。制备单柱采用C18为固定相,乙醇水为流动相,采用50%乙醇、70%乙醇及纯乙醇梯度洗脱,洗脱馏分经浓缩得到适合异甘草素分离的模拟移动床进样原料。
Using orthogonal test combined with single column separation, the raw materials of licorice flavonoids were pretreated. The optimum extraction conditions obtained by orthogonal experiment were as follows: the mass concentration of raw material was 30g / L, the solvent was extracted by ethanol, the extraction was performed by ultrasonic for 60min, the extraction temperature was 20 ℃ and the concentration of extract was 10g / L. The single-column was prepared with C18 as stationary phase and ethanol water as mobile phase. The mobile phase was eluted with 50% ethanol, 70% ethanol and pure ethanol. The eluted fractions were concentrated to obtain simulated moving bed sample material suitable for the separation of isoliquiritigenin.