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目的:利用体外细胞培养技术,对聚乳酸-壳聚糖接骨板的细胞毒性进行研究,为临床应用作前期准备。方法:采用间接接触法(MTT法)评价聚乳酸-壳聚糖接骨板的细胞毒性。MTT法:采用体外培养的小鼠成纤维细胞(L929),在培养液中分别添加不同浓度的聚乳酸-壳聚糖接骨板浸提液(0,50%和100%),逐日用倒置显微镜对各组细胞进行形态学观察,于接种后2、4、7 d通过MTT法显色,在酶标仪450 nm波长处测定吸光度值,计算相对增殖率,对聚乳酸-壳聚糖接骨板的细胞毒性进行评价。结果:随时间推移,不同浓度聚乳酸-壳聚糖接骨板浸提液对L929细胞增殖均有促进作用(P<0.01),在2、4、7 d各浓度之间无明显差异性(P>0.05),提示L929细胞增殖与聚乳酸-壳聚糖接骨板浸提液的浓度无明显相关;随时间推移,实验各组L929细胞数量、形态学变化与空白对照组无明显差异,评分聚乳酸-壳聚糖接骨板的细胞毒性为0级。结论:聚乳酸-壳聚糖接骨板无明显细胞毒性,具有良好细胞相容性,是一种具有发展前景的可吸收骨折内固定材料。
OBJECTIVE: To study the cytotoxicity of poly (L-lactic acid) -chitosan bone plate by in vitro cell culture technique, and to prepare for clinical application. Methods: The cytotoxicity of polylactide - chitosan plate was evaluated by indirect contact method (MTT). MTT method: Using mouse fibroblasts cultured in vitro (L929), different concentrations of polylactic acid-chitosan bone plate extract (0, 50% and 100%) were added to the culture medium, The morphology of each group of cells was observed, MTT assay was performed on the 2nd, 4th and 7th day after inoculation, the absorbance was measured at a wavelength of 450 nm and the relative proliferation rate was calculated. The polylactic acid-chitosan plate The cytotoxicity was evaluated. Results: Over time, the polylactic acid-chitosan plate extracts promoted the proliferation of L929 cells (P <0.01), and there was no significant difference between the concentrations of P > 0.05), suggesting that the proliferation of L929 cells and polylactic acid - chitosan platelet concentration was not significantly related; over time, experimental L929 cells in each group, the number of morphological changes and the control group no significant difference, The cytotoxicity of lactic acid-chitosan plate was level 0. CONCLUSION: Polylactic acid-chitosan plate has no obvious cytotoxicity and good cytocompatibility. It is a prospective absorbable fracture fixation material.