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体内扩散盒(Diffusion chamber)培养可使细胞在较接近自然的情况下生长。本文动态地观察了4株小鼠白血病(L615、L7212、L1210、P388)细胞在扩散盒中生长的过程。培养6～7天,细胞经对数生长期后可达生长高峰,细胞数可为植入时的5～14倍。如果使受鼠口服或肌注抗癌药物,可影响扩散盒内细胞的生长,其作用与肿瘤细胞对抗癌药物的敏感性有关。 本文研究了体内扩散盒培养的P388细胞对11种抗癌药物的敏感性,其中体内给药有效(生命延长率>25％)者7种,无效者4种。结果除6-巯基嘌呤的生命延长率为33％,而扩散盒培养细胞的生长抑制率为13.3％外(>35％为有效),其余均相符合。 本方法将抗癌药物筛选的体内、外实验法的优缺点结合起来考虑,国内外尚未见报道。它具有能综合反映药物对机体毒性、在体内代谢情况以及肿瘤细胞对药物敏感性的特点,并可用于培养人肿瘤细胞。对于寻找人肿瘤细胞敏感的抗癌药物,指导临床用药以及探索药物作用机理具有一定价值,从而为抗癌药物的筛选提供了一个新的途径。
Diffusion chamber culture allows cells to grow closer to nature. This article dynamically observed the growth of 4 murine leukemia (L615, L7212, L1210, P388) cells in a diffusion box. After cultured for 6 to 7 days, the cells reached the peak of growth after logarithmic growth phase, and the number of cells could be 5 to 14 times of the time of implantation. If administered orally or intramuscularly, the anti-cancer drugs can affect the growth of the cells in the diffusion box, which is related to the sensitivity of tumor cells to anticancer drugs. In this paper, the proliferation of P388 cells in vitro proliferation of 11 kinds of anti-cancer drugs sensitivity, which was administered in vivo (life-span> 25%) were 7, 4 were ineffective. Results The life prolongation of 6-mercaptopurine was 33%, while the growth inhibition rate of diffusion cell cultured cells was 13.3% (> 35% was effective), the rest were consistent. The method combines the advantages and disadvantages of in vitro and in vivo experimental methods for screening anticancer drugs, and has not been reported at home and abroad. It has a comprehensive reflection of the toxicity of drugs on the body, in vivo metabolism and the characteristics of tumor cells on drug sensitivity, and can be used to culture human tumor cells. It is of great value to find anticancer drugs sensitive to human tumor cells, to guide the clinical use of drugs and to explore the mechanism of drug action, thus providing a new way for the screening of anticancer drugs.