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目的探讨十八烯酸对人肾癌细胞A498细胞的毒性作用。方法从甘肃地产猫儿眼(Euphorbia kansui L)提取并制备十八烯酸。给予人A498细胞0.3、0.6、3.0 mg/L十八烯酸培养,分别采用MTT法、克隆形成和流式细胞技术对十八烯酸对人A498细胞的毒性作用进行检测。结果给予人A498细胞0.3、0.6、3.0 mg/L十八烯酸,细胞增殖抑制率随给药浓度增加而增加(分别为53.20%、66.26%、87.42%),半数抑制浓度(IC50)为0.29 mg/L;克隆形成抑制率随给药浓度增加而增加(分别为46.43%、61.31%、71.42%),IC50为0.31 mg/L;人A498细胞凋亡率别为11.16%、17.31%、26.22%。结论十八烯酸对人A498细胞具有明显的增殖抑制作用,能促进人A498细胞凋亡。
Objective To investigate the toxic effect of octadecanoic acid on human renal cell carcinoma A498 cells. Methods Oleic acid was extracted from Euphorbia kansui L and prepared from Gansu real estate. The human A498 cells were treated with 0.3,0.6,3.0 mg / L octadecenoic acid. The toxic effects of octadecenoic acid on human A498 cells were detected by MTT method, colony formation and flow cytometry respectively. The results showed that 0.3,0.6 and 3.0 mg / L of octadecenoic acid were administered to human A498 cells, and the rate of inhibition of cell proliferation increased with increasing concentration (53.20%, 66.26% and 87.42%, respectively). The IC50 was 0.29 mg / L. The inhibition rate of colony formation increased with the increase of drug concentration (46.43%, 61.31%, 71.42%, respectively) and the IC50 was 0.31 mg / L. The apoptotic rates of human A498 cells were 11.16%, 17.31%, 26.22 %. Conclusion Octapenic acid has obvious proliferation inhibitory effect on human A498 cells and can promote the apoptosis of human A498 cells.