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目的了解云南省部分地区虫媒病毒的存在及流行情况。方法2002年和2004年从云南省5个县市采集蚊虫,经研磨、细胞接种分离病毒,通过实时荧光PCR、免疫荧光试验等方法进行鉴定,并对病毒的分子生物学特性进行分析。结果采集到4810只蚊虫,分离到12株致BHK细胞病变的病毒,经实时荧光PCR、免疫荧光试验等鉴定为乙脑病毒。新分离病毒DL0437属于基因Ⅲ型乙脑病毒。结论2002年和2004年在云南5个县市采集4810只蚊虫中分离到12株乙脑病毒,是近年来在云南省首次分离到基因Ⅲ型乙脑病毒。
Objective To understand the prevalence and prevalence of arbovirus in some areas of Yunnan Province. Methods In 2002 and 2004, mosquitoes were collected from 5 counties in Yunnan Province. The viruses were isolated by grinding and cell inoculation. The viruses were identified by real - time fluorescence PCR and immunofluorescence assay. The molecular biological characteristics of the viruses were also analyzed. Results A total of 4810 mosquitoes were collected and 12 strains of BHK cells were isolated and identified as JE virus by real-time fluorescence PCR and immunofluorescence assay. The new isolated virus DL0437 belongs to genotype III JE virus. Conclusions 12 JE viruses were isolated from 4810 mosquitoes collected in 5 counties and cities in Yunnan Province in 2002 and 2004, and JEV was isolated for the first time in Yunnan Province in recent years.