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目的 :通过观察人结肠癌LoVo细胞裸鼠移植瘤bcl 2及bax基因表达水平 ,探讨脂肪酸合酶抑制剂浅蓝菌素选择性的抑瘤途径和机制。方法 :建立人结肠癌裸鼠移植瘤模型 ,浅蓝菌素每公斤体重 160mg腹腔注射 ,1次·d-1,连续10d ,第 17天处死并解剖动物 ,瘤组织作免疫组织化学检测bcl 2及bax基因表达水平。结果 :浅蓝菌素处理组结肠癌LoVo细胞移植瘤bcl 2基因蛋白表达率为 68.5 % ;对照组bcl 2蛋白阳性表达率为 87.8% ,阳性细胞弥漫分布。两组间有显著性差异 (P <0 .0 1)。bax基因蛋白在浅蓝菌素组移植瘤组织阳性表达率为 75 .4% ,主要呈强阳性表达 ;对照组阳性表达率为 9.5 %。两组间比较有统计学差异 (P <0 .0 1)。结论 :浅蓝菌素可使结肠癌LoVo细胞移植瘤的bcl 2基因表达下调 ,bax基因表达增强 ,提示bcl 2及bax基因在浅蓝菌素诱导的人结肠癌LoVo细胞凋亡中可能起着重要的调控作用。
Objective : To investigate the selective tumor inhibition pathway and mechanism of cysaretin, a fatty acid synthase inhibitor, by observing the expression levels of bcl 2 and bax genes in transplanted tumors of human colon cancer LoVo cells. METHODS: A human colon cancer xenograft model of nude mice was established. The cerulenin was injected intraperitoneally with 160 mg per kilogram of body weight once every 1 d for 10 consecutive days. The animals were sacrificed and dissected on the 17th day. The tumor tissues were used for immunohistochemical detection of bcl 2 And bax gene expression levels. RESULTS: The expression of bcl-2 gene in colon cancer colon cancer LoVo cells was 68.5% in the cerulenin-treated group and 87.8% in the control group. The positive cells were diffusely distributed. There was a significant difference between the two groups (P < 0.01). The positive expression rate of bax gene protein in the cerulenin group was 75.4%, which was mainly positive expression. The positive expression rate of the control group was 9.5%. There was a statistically significant difference between the two groups (P < 0.01). Conclusion: Cerulenin can down-regulate the expression of bcl 2 gene in colon cancer LoVo cells and increase the expression of bax gene, suggesting that bcl 2 and bax genes may play a role in cerulenin-induced apoptosis of human colon cancer LoVo cells. Important regulatory role.