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目的：探索ＨＣＶＤＮＡ核酸免疫效应。方法：将构建的重组体ｐＣＤＨＣＶｓ免疫Ｂａｌｂ／ｃ小鼠，用ＥＬＩＳＡ和３ＨＴｄＲ掺入法检测了免疫鼠血清抗体、脾细胞和ＰＢＭＣ对ＨＣＶ抗原的增殖反应。结果：重组体ｐＣＤＨＣＶ１、ｐＣＤＨＣＶ２和ｐＣＤＨＣＶ３均可诱导小鼠（ｎ＝６）产生抗体（０．６６５～０．７０７，ＯＤ值）和脾细胞对ＨＣＶ抗原增殖反应（ｎ＝６，ＳＩ为３．８９～４．４７），但各重组体间的免疫效应无显著性差异（Ｐ＞０．０５）；免疫鼠（ｎ＝１２）血清经４０倍和３２０倍稀释后，抗体阳性率分别为１００．０％和１６．６％；重组体还可诱导鼠ＰＢＭＣ对ＨＣＶ抗原的增殖反应（３．６９～４．０７，ＳＩ）。结论：ｐＣＤＨＣＶｓ重组体可诱导小鼠产生免疫反应，该结果对进一步开展ＨＣＶＤＮＡ疫苗研制将具有重要意义。
Objective: To explore the effect of HCV DNA immunization. Methods: Balb / c mice were immunized with the constructed recombinant pCD-HCV and the proliferative responses of the immunized rat serum, spleen cells and PBMCs to HCV antigens were detected by ELISA and 3H-TdR incorporation. RESULTS: Recombinant pCDHCV1, pCDHCV2 and pCDHCV3 could induce antibody (0.665 ~ 0.707, OD value) in mouse (n = 6) and proliferative response of splenocytes to HCV antigen (n = 6, SI was 3.89 ~ 4.47), but there was no significant difference in the immunological effect between the recombinant plasmids (P> 0.05). After 40 times and 320 times dilutions of the immunized mice (n = 12) The antibody positive rates were 100.0% and 16.6% respectively. Recombinant also induced the proliferative response of mouse PBMC to HCV antigen (3.69 ~ 4.07, SI). Conclusion: Recombinant pCD-HCV can induce immune response in mice, the results of further development of HCV DNA vaccine will be of great significance.