论文部分内容阅读
目的探讨LIGHT对利什曼原虫(L.major)感染模型中CD4+T细胞亚群免疫应答的影响。方法采用LIGHT KO和野生型(WT)小鼠,经小鼠脚掌注射L.major建立感染模型。从感染第2周至24周连续观察被感染脚掌的病损程度,并测定感染24周小鼠体内寄生虫载量。其次,取感染第7天小鼠引流淋巴结(d LN)细胞,运用3H-Td R掺入方法检测T细胞体外刺激的增殖情况,并且利用ELSIA检测体外刺激后d LN细胞IL-12和IFN-γ的表达水平。最后,通过流式细胞术检测感染第7天小鼠脾脏和d LN中Treg水平。结果 LIGHT KO小鼠被感染脚掌和d LN的寄生虫载量均显著高于WT对照小鼠。感染早期LIGHTKO小鼠d LN中T细胞增殖水平,以及IL-12和IFN-γ的表达水平均显著低于WT对照小鼠。感染早期LIGHT KO小鼠中脾脏和d LN中的Treg细胞百分率相对WT小鼠均显著升高。结论 LIGHT可能通过抑制Treg细胞的产生,促进Th1细胞的分化,从而增强小鼠抗L.major感染的能力。
Objective To investigate the effect of LIGHT on the immune response of CD4 + T lymphocyte subsets in L. major infection model. Methods LIGHT KO and wild-type (WT) mice were used to establish an infection model by injecting L. major with mouse paw. From the second week to the 24th week of infection, the degree of lesion of infected feet was observed continuously and the parasite load of mice in 24 weeks after infection was determined. Then, the proliferation of T lymphocytes in vitro was detected by 3H-TdR incorporation method, and the expression of IL-12 and IFN-γ in d LN cells was detected by ELSIA. γ expression level. Finally, Treg levels in spleen and d LN of mice on day 7 of infection were detected by flow cytometry. Results The parasite burden of litter and d LN in LIGHT KO mice was significantly higher than WT control mice. The level of T cell proliferation and the expression of IL-12 and IFN-γ in d LN of early infected LIGHTKO mice were significantly lower than WT control mice. The percentage of Treg cells in spleen and d LN in early infected LIGHT KO mice was significantly higher than that in WT mice. Conclusion LIGHT may promote the differentiation of Th1 cells and enhance the ability of anti-L. major infection in mice by inhibiting the production of Treg cells.