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本研究旨在检测白血病细胞株及其耐药株中两种药物代谢相关基因的单核苷酸多态性。培养白血病细胞株K562及其耐药株K562/A02,采用QIAamp DNA Blood Minikit试剂盒提取基因组DNA,设计引物,应用PCR技术扩增相应目的片段;采用基于基质辅助激光解吸电离飞行时间质谱技术检测mthfr基因rs1801131、rs1801133、rs2274976及dpyd基因rs1801159、rs1801160、rs17376848的单核苷酸多态性。结果表明,在K562和K562/A02两种细胞中,mthfr基因rs1801131基因型均为CA、rs1801133均为CC、rs2274976均为GG;dpyd基因rs1801159基因型均为GG、rs1801160均为GG、rs17376848均为AA。结论:mthfr、dpyd基因上述位点在K562和K562/A02两种细胞中基因表达无差别。
This study was designed to examine single nucleotide polymorphisms of two drug metabolism related genes in leukemia cell lines and drug-resistant strains. The leukemia cell line K562 and its resistant strain K562 / A02 were cultured and the genomic DNA was extracted with QIAamp DNA Blood Minikit kit. Primers were designed and amplified by PCR. The mthfr Rs1801131, rs1801133, rs2274976 and dpyd rs1801159, rs1801160, rs17376848 single nucleotide polymorphisms. The results showed that the mthfr gene rs1801131 genotype CA, rs1801133 are CC, rs2274976 are GG; dpyd gene rs1801159 genotype GG, rs1801160 are GG, rs17376848 are AA. Conclusion: There is no difference in gene expression between mthfr and dpyd genes in K562 and K562 / A02 cells.