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在色谱柱为 Supelcosil SPLC-18-DB(5μ,250×10mm),流动相为含 5mM四丁基溴化铵的0.02M磷酸二氢钾缓冲溶液和乙腈(75:25,v/v),流速为1.0ml/min,柱温为30℃的条件下,7种葡萄苷酸内酯盐成分得到完全的分离,其中4种主要成分被定量测定。此法具有简便、快速、准确性和重现性好的优点。文中对主要的色谱参数(流动相的组成、pH值、柱温)对保留值和选择性的影响也进行了较详细的讨论。
The column was Supelcosil SPLC-18-DB (5μ, 250 × 10 mm), the mobile phase was 0.02 M potassium dihydrogen phosphate buffer containing 5 mM tetrabutylammonium bromide and acetonitrile (75:25, v / v) Flow rate of 1.0ml / min, the column temperature of 30 ℃ conditions, seven kinds of glucuronolactone salt components were completely separated, of which four kinds of main components were quantitatively determined. This method has the advantages of simplicity, speed, accuracy and reproducibility. The effects of the main chromatographic parameters (mobile phase composition, pH, column temperature) on retention and selectivity are also discussed in more detail.