论文部分内容阅读
目的 :探讨IL 4和IL 12在呼吸道合胞病毒 (RSV)引起的下呼吸道感染中所起的作用。方法 :①用碱性磷酸酶抗碱性磷酸酶桥联酶标法 (APAAP)检测出 30例RSV抗原阳性患儿 ;②应用双抗体夹心酶联免疫吸附法 (ELISA)分别检测患儿和正常儿童血清中IL 4、IL 12水平并进行相关分析。结果 :①治疗前RSV下呼吸道感染患儿血清IL 4水平显著升高 ,IL 12水平显著降低 ,与对照组比较有统计学意义 (P <0 .0 0 5 )。②治疗后病情好转患儿的IL 4水平下降 ,IL 12水平升高 ,与正常对照无显著性差别 (P >0 .0 5 )。③血清IL 4、IL 12之间有显著负相关关系 (r =-0 .432 ,P <0 .0 5 )。结论 :RSV下呼吸道感染存在Th1/Th2功能紊乱 ,主要表现Th2细胞功能亢进 ,IL 4、IL 12参与了RSV下呼吸道感染时的免疫病理机制。
Objective: To investigate the role of IL 4 and IL 12 in lower respiratory tract infection caused by respiratory syncytial virus (RSV). Methods: ①30 cases of RSV antigen positive children were detected by alkaline phosphatase-alkaline phosphatase-linked enzyme-linked assay (APAAP); ②The antibody levels in normal children and normal children were detected by enzyme-linked immunosorbent assay (ELISA) IL-4 and IL-12 levels in children’s serum were analyzed. Results: ① The level of IL-4 in serum of children with RSV lower respiratory tract infection before treatment was significantly increased, and the level of IL-12 was significantly decreased compared with the control group (P <0.05). (2) IL-4 level decreased and IL-12 level increased in children with improved condition after treatment. There was no significant difference between the two groups (P> 0.05). ③ There was a significant negative correlation between serum IL 4 and IL 12 (r = -0.432, P <0.05). CONCLUSIONS: Th1 / Th2 dysfunction occurs in RSV lower respiratory tract infection, which mainly indicates the hyperactivity of Th2 cells. IL 4 and IL 12 are involved in the immunopathological mechanism of RSV lower respiratory tract infection.