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为研究类风湿关节炎时关节滑膜浸润性T细胞生物学特性与致病机制 ,对 10例RA患者滑膜液中淋巴细胞的免疫表型、细胞因子分泌格局与趋化因子受体表达进行了分析。用双色荧光标记法分别测定滑膜液中和外周血淋巴细胞表型与趋化因子受体表达。用ELISA方法检测滑膜液与外周血中IFN γ、IL 10、IL 4与IL 12的含量。结果是滑膜液中的CD4 + T淋巴细胞为 4 0 0 %± 11% ,CD8+ T细胞为 34 0 %± 6 % ,CD4 + 与CD8+ T细胞的比值为 1 2 ,显著低于外周血中CD4 /CD8的比值。滑膜液中CD3和CD2 5双阳性的活化T细胞占 16 %± 6 0 %。趋化因子受体CCR5表达较低 ,与外周血无明显差异。但CX CR3表达水平较高 ,为 16 %± 4 0 % ,远远高于外周血 (仅为 0 5 %± 0 3% )。IFN γ在滑膜液中含量很高 ,达 (36 6 7± 4 3 2 )pg/ml,而外周血中含量仅为 (2 0 1± 3 2 )pg/ml。IL 4含量未能测得 (<15pg/ml ) ,与外周血相似。IL 12含量为 (4 19 9±89 2 )pg/ml,远高于外周血中的含量 (6 5 32± 34 2 )pg/ml。IL 10含量为 (187 7± 34 5 )pg/ml,高于外周血中的含量 (85±12 7)pg/ml。在所测细胞因子中 ,关节滑膜液中IFN γ和IL 12的含量与外周血相比具有显著的统计学差异。表明RA关节滑膜液中有相当数量的T细胞浸润。这些T细胞
In order to study the biological characteristics and pathogenesis of synovial infiltrating T cells in rheumatoid arthritis, the immunophenotype, cytokine secretion pattern and chemokine receptor expression of lymphocytes in synovial fluid of 10 RA patients were studied Analysis. The phenotypes and chemokine receptor expression in synovial fluid and peripheral blood lymphocytes were measured by two-color fluorescent labeling. The contents of IFN-γ, IL-10, IL-4 and IL-12 in synovial fluid and peripheral blood were detected by ELISA. As a result, the percentage of CD4 + T lymphocytes in synovial fluid was 400% ± 11%, the percentage of CD8 + T cells was 34% ± 6%, the ratio of CD4 + to CD8 + T cells was 12, which was significantly lower than that in peripheral blood CD4 / CD8 ratio. Synovial fluid CD3 and CD2 5 double-positive activated T cells accounted for 16% ± 60%. Chemokine receptor CCR5 expression is low, no significant difference with peripheral blood. However, CXCR3 expression was high at 16% ± 40%, much higher than that in peripheral blood (only 0 5% ± 0 3%). IFNγ was found to be high in synovial fluid (36 6 7 ± 4 3 2) pg / ml, whereas peripheral blood was only (2 0 1 ± 3 2) pg / ml. IL 4 levels were not measured (<15 pg / ml), similar to peripheral blood. The content of IL 12 was (4 19 9 ± 89 2) pg / ml, which was much higher than that in peripheral blood (6 5 32 ± 34 2) pg / ml. IL 10 content was (187 7 ± 34 5) pg / ml, higher than that in peripheral blood (85 ± 12 7) pg / ml. Among the cytokines tested, the levels of IFN? And IL? In synovial fluid were significantly different from those in peripheral blood. RA joint synovial fluid that a significant amount of T cell infiltration. These T cells