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参照国外发表的恙虫病立克次体Karp株sta58全基因序列,合成1对DNA引物(27个碱基和23个碱基),以Karp DNA为模板,成功扩增了长约Ikb的DNA片段。将PCR技术应用于立克次体研究,为今后用PCR检测立克次体以及立克次体分子克隆研究起了探索作用。
A pair of DNA primers (27 bases and 23 bases) was synthesized with reference to the sta58 gene sequence of Rickettsia tsutsugamushi Karp strain in the world. The DNA fragment of about 1 kb in length was successfully amplified using Karp DNA as a template . The application of PCR technology to Rickettsia studies for the future detection of Rickettsia rickettsia and Rickettsia molecular cloning played a role in exploration.