丝线、羊肠线及结扎道数对大鼠CCI疼痛模型的影响

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通过比较丝线、羊肠线2种不同材料和不同结扎道数对大鼠CCI疼痛模型痛阈值的影响,优化筛选出更易在实验过程中普及的技术方法 .将42只SD雄性大鼠随机分成7组,包括结扎丝线4道、3道与2道3个组;结扎羊肠线4道、3道与2道3个组和1个假手术组,每组6只大鼠.铬制羊肠线和丝线分别结扎大鼠左侧坐骨神经2道、3道、4道;假手术组只暴露左侧坐骨神经,不进行结扎处理.建模成功后测量各组大鼠的机械性缩足反应阈值(MWT),发现丝线3道、4道组和羊肠线2道、3道、4道组与假手术组和自身基础的MWT有极显著差异(P<0.01);假手术对照组、丝线2道组与各组自身基础的MWT之间没有差异(P>0.05),结扎手术前后的MWT之间差异显著,结扎后第7天、14天和21天的MWT之间没有差异.羊肠线3组之间没有显著性差异(P>0.05).由此可得出结论:羊肠线2道、3道、4道组,以及丝线3道、4道组均能产生稳定的神经痛,根据MWT的数值大小和是否出现自噬现象可认为,以羊肠线结扎2道组最好,不但MWT的数值小,且操作简便又节省时间,可作为大鼠CCI建模的首选方法 . By comparing the effects of two different materials, such as silk thread, catgut, and different ligation number on the pain threshold of CCI pain model in rats, the technical methods that are easier to be popularized during the experiment were screened.All 42 SD male rats were randomly divided into 7 Group, including ligation silk 4, 3 and 2 3 groups; ligation of catgut 4, 3 and 2 3 groups and 1 sham group, 6 rats in each group. The left sciatic nerve was ligated with 2, 3 and 4 sciatic nerves in the left and right sides of the sciatic nerve in sham-operation group, while the left sciatic nerve was not ligated in the sham-operated group.The mechanical contractions threshold MWT). The results showed that there were significant differences (P <0.01) between the sham-operated group and the MWT of the basilar wounds in the 3rd, 4th and the 2nd, There was no significant difference between the MWT of baseline group and each group (P> 0.05), there was significant difference between the MWT before and after ligation, there was no difference between MWT on the 7th, 14th and 21st days after ligation There was no significant difference between the three groups (P> 0.05) .Therefore, it can be concluded that: catgut 2, 3, 4, and silk 3, 4 groups were stable According to the value of MWT and the phenomenon of autophagy, we can conclude that it is the best way to ligate 2 groups with catgut, not only the value of MWT is small, but also easy to operate and save time, which can be used as the first choice of modeling of CCI in rats Method.
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