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目的研究空间微重力环境对电泳分离的影响,证实自主研制的A3-2型电泳仪的设计和工艺,为我国空间电泳研究的发展和生物材料加工及空间制药奠定基础。方法用A3-2型连续自由流电泳仪,分别在地面和空间对细胞色素C和牛血红蛋白两种模式蛋白进行电泳分离实验,对其结果进行分析比较。结果电泳仪在“神舟”4号飞船上成功地进行了空间电泳分离实验,并获得了完整的飞行试验数据。返回舱着陆后,回收的电泳仪装置完好无损,得到了全部(30管)分离产物。结论在线光电检测及分离产物的检测结果都说明,空间微重力环境下的电泳分离效果明显优于地面的结果。飞行试验表明,A3-2电泳仪的结构设计和工艺是正确和可行的,它为我国首次进行的空间电泳分离实验取得圆满成功提供了保障。
Objective To study the influence of spatial micro-gravity environment on electrophoretic separation and confirm the design and technology of self-developed A3-2 electrophoresis apparatus, which lays the foundation for the development of space electrophoresis research and biomaterial processing and space medicine in our country. Methods Two types of proteins, cytochrome C and bovine hemoglobin, were electrophoretically separated on the ground and in space using A3-2 continuous free-flow electrophoresis. The results were analyzed and compared. Results The electrophoresis instrument successfully carried out the space electrophoresis separation experiments on the “Shenzhou” spacecraft 4 and got the complete flight test data. After landing on the capsule, the recovered electrophoresis device was left intact and all (30 tubes) separated. Conclusion The results of on-line photodetector and separation products show that the separation effect of electrophoresis in space microgravity environment is better than that of ground. Flight tests showed that the structural design and process of A3-2 electrophoresis apparatus were correct and feasible, which provided the guarantee for the first successful space electrophoresis separation experiment in our country.