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目的调查浙江省舟山医院就诊的女性宫颈炎患者人乳头瘤病毒(HPV)检出率及基因型分布,为本地女性HPV感染控制提供基础数据。方法采用流式荧光杂交技术检测女性宫颈炎患者的宫颈脱落细胞样本HPV并进行基因分型。结果 997例宫颈炎受检者中,HPV DNA阳性者220例,HPV检出率为22.1%(220/997)。高危型、低危型和多重HPV检出率分别为13.8%(138/997)、0.4%(4/997)和7.8%(78/997)。共检出19种HPV基因型。高危型HPV检出率居前5位的基因型依次是HPV 52型占8.4%(84/997)、58型4.9%(49/997)、16型3.3%(33/997)、18型2.2%(22/997)和33型1.9%(19/997);低危型HPV检出率最高的亚型是HPV 6型1.6%(16/997)。不同年龄组人群HPV检出率差异有统计学意义(P<0.05),以31~40岁年龄组HPV检出率最高,为8.1%(81/997)。结论舟山海岛地区HPV感染在基因型及年龄分布上具有一定的特点,与其他地区存在差异。
Objective To investigate the detection rate and genotype distribution of human papillomavirus (HPV) in female cervicitis patients in Zhoushan Hospital of Zhejiang Province, and provide the basic data for the control of HPV infection in local women. Methods Flow cytometry (FCM) was used to detect HPV and genotypes of cervical exfoliated cells from female patients with cervicitis. Results Among the 997 cervicitis patients, 220 cases were positive for HPV DNA, the detection rate of HPV was 22.1% (220/997). High-risk, low-risk and multiple HPV detection rates were 13.8% (138/997), 0.4% (4/997) and 7.8% (78/997), respectively. A total of 19 HPV genotypes were detected. The top 5 genotypes of high-risk HPV were HPV 52 type (8.4%), type 49 4.9% (49/997), type 16 3.3% (33/997), type 18 2.2 % (22/997) and 33 (1.9%) (19/997) respectively. The highest subtype of low-risk HPV was 1.6% (16/997) of HPV type 6. The detection rate of HPV among different age groups was significantly different (P <0.05). The highest HPV detection rate was 8.1% (81/997) in 31-40 years old group. Conclusion HPV infection in Zhoushan island area has certain characteristics in genotype and age distribution, which is different from other regions.