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为了研究氧化修饰对高密度脂蛋白 (HDL)转运细胞胆固醇的影响 ,将3 H 胆固醇负荷的培养人动脉平滑肌细胞 (SMC)分别与天然HDL及Cu2 + 或HOCl氧化修饰的HDL在 37℃温育不同时间后 ,分别测定细胞3 H 胆固醇清除率。结果发现 ,温育 2 4h后 ,经Cu2 + 或HOCl氧化修饰后的HDL其细胞胆固醇清除率分别较天然HDL下降了 30 .0 %和 43.1% (p <0 .0 1)。结果还发现 ,Cu2 + 或HOCl氧化修饰的HDL其转运细胞胆固醇的速率也比天然HDL大大降低。加入叔丁基甲苯 (BHT)、VitC及VitE可抑制Cu2 + 或HOCl诱导HDL的氧化修饰 ,使其转运细胞胆固醇的能力提高。
To investigate the effect of oxidative modification on HDL transporter cholesterol, 3 H cholesterol-loaded cultured human smooth muscle cells (SMC) were incubated with native HDL and Cu2 + or HOC1 oxidized HDL at 37 ° C After different times, the 3 H cholesterol clearance rate of cells was measured. The results showed that after 24 h of incubation, the HDL cholesterol levels decreased by 30.0% and 43.1% (p <0.01), respectively, compared with the native HDL after Cu2 + or HOC1 oxidation. The results also found that, Cu2 + or HOCl oxidatively modified HDL its rate of cellular cholesterol transport also significantly lower than the native HDL. The addition of tert-butyltoluene (BHT), VitC and VitE can inhibit the oxidative modification of HDL induced by Cu2 + or HOCl and increase their ability to transport cellular cholesterol.