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鱼类快肌和慢肌分别占据骨骼肌的不同位置,表现不同的生长发育特征。为了解鳜(Sinipercachuatsi)慢肌纤维的胚后发育特征,本研究通过制作孵化后1～33日龄鳜个体的石蜡切片,采用慢肌特异抗体的免疫组织化学染色,观察了背鳍起点处躯干横切面慢肌的发育变化特征,并利用图像分析软件统计慢肌纤维的数目和面积。结果表明,孵化后鳜仔鱼慢肌位于水平肌隔附近,呈楔形,向背、腹两侧生长。孵化后1～9日龄为单层肌纤维,11日龄发育为多层肌纤维,19日龄覆盖侧线附近,33日龄延伸至背侧第2背肌节、腹侧腹部肌肉2/3处,并在水平肌隔和侧线处分别形成两个肌群。位于骨骼肌最外层的扁平状表层细胞,可能为慢肌增生生长的主要来源。躯干单侧慢肌肌纤维数目由孵化后6个增加至315个,总面积从13.18μm2增加到7 839.58μm2,孵化后13日龄的增生生长占优势,其他发育阶段,肥大生长一直占主导优势。
Fast and slow muscle fish occupy different positions of skeletal muscle, showing different growth and development characteristics. In order to understand the post-embryonic development characteristics of Sinipercachuatsi slow muscle fibers, we used immunohistochemical staining of slow-muscle-specific antibodies to make paraffin sections of individuals aged 1 to 33 days after hatching, Slow muscle development and changes in the characteristics, and the use of image analysis software to count the number and area of slow muscle fibers. The results showed that after larval larval larval larvae located in the level of muscle around the diaphragm, was wedge-shaped, back and abdomen growth. After hatching 1 to 9 days of age for the monolayer muscle fibers, 11 days of age developed into multi-muscle fibers, 19 days to cover the lateral line near the 33-day extension to the dorsal dorsalis medulla, ventral abdominal muscle 2/3, And in the horizontal muscle and lateral line at the formation of two muscle groups. Flat surface cells located in the outermost layer of skeletal muscle may be the main source of slow muscle hyperplasia. The number of unilateral slow muscle fibers increased from 6 to 315 after hatching, and the total area increased from 13.18μm2 to 7 839.58μm2. The 13-day-old proliferative growth predominates after hatching. In other developmental stages, the growth of hypertrophic fetus has predominated.