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目的:探讨Survivin表达对肺鳞癌细胞的凋亡和增殖的影响。方法:利用siRNA阻抑人肺鳞癌细胞内survivin基因的表达,用RT-PCR和Western Blotting法分析survivin基因mRNA和蛋白的表达,流式细胞术检测细胞凋亡率,细胞集落形成实验检测细胞增殖。结果:(1)Survivin在肺癌细胞中表达,转染Survivin siRNA可在RNA和蛋白水平阻断其表达;(2)转染Survivin siRNA的肺癌细胞凋亡率显著增加;(3)转染Survivin siRNA的肺癌细胞的集落形成率显著降低。结论:阻断Survivin表达可通过增加细胞凋亡率和降低细胞增殖增加肺鳞癌细胞的放疗敏感性。
Objective: To investigate the effect of Survivin expression on the apoptosis and proliferation of lung squamous cell carcinoma. Methods: The expression of survivin gene in human lung squamous cell carcinoma cell line was inhibited by siRNA. The expression of survivin mRNA and protein was analyzed by RT-PCR and Western Blotting. The apoptosis rate was detected by flow cytometry. The cell colony forming assay proliferation. Survivin siRNA was transfected into lung cancer cells. Survivin siRNA transfected Survivin siRNA could block the expression of Survivin at the level of RNA and protein. (2) Of lung cancer cells significantly reduced the colony formation rate. Conclusion: Blocking Survivin expression can increase the radiosensitivity of lung squamous cell carcinoma cells by increasing the apoptosis rate and decreasing the cell proliferation.