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采用沉淀聚合法制备了加替沙星分子印迹聚合物(MIP)微球,对其形貌和结构进行表征,并对聚合反应条件进行优化;采用静态吸附试验研究聚合物对模板分子及其结构类似物的吸附行为和选择性识别特性.结果表明,在最佳实验条件下,40 min印迹聚合物即可达到对底物的吸附平衡;在印迹聚合物中存在两类结合位点,特异性和非特异性结合位点的最大吸附量分别为363和458μmol/g;与化学组成相同的非印迹聚合物(NIP)相比,MIP对结构相似的喹诺酮类抗生素具有良好的选择吸附性能;该法制备的MIP微球易于再生,放置3个月以后,仍能够对喹诺酮类抗生素进行有效识别.
The microspheres of gatifloxacin molecularly imprinted polymer (MIP) were prepared by precipitation polymerization and the morphology and structure of the microspheres were characterized and the polymerization conditions were optimized. The static adsorption experiments were carried out to study the effects of the polymer on the template molecule and its structure The results showed that under the optimal experimental conditions, the adsorption equilibrium of the substrate could be achieved after 40 min imprinted polymer. There were two kinds of binding sites in the imprinted polymer, the specificity And nonspecific binding sites were 363 and 458 μmol / g, respectively. Compared with non-imprinted polymer (NIP) of the same chemical composition, MIP had good selective adsorption capacity for quinolone antibiotics with similar structure. The prepared MIP microspheres are easy to regenerate, and after being placed for 3 months, the quinolone antibiotics can still be effectively identified.