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以~(14)C-氯代乙酸钠制备~(14)C-增产灵;以 Na~(125)I制备~(125)I-增产灵。在孕穗期或湖浆初期,采取叶面涂布、喷施或淋浇根部方法,将标记物分别引进水稻体内。 涂布~(125)I-增产灵(1000 ppm,0.1毫升)于剑叶面3小时后,标记物在叶片中已达附着量的75.2％;一部分标记物并从叶片传递至叶鞘、茎、穗及其它叶片,其中以叶鞘的积累量较多。 叶面喷施~(125)I-增产灵(100 ppm),l小时内渗入量为附着量的26.5％,随时间延长,吸收量增加。标记物在叶片积累量最多,叶鞘次之,运入茎与穗很少,加入0.1％肥皂液可增加叶面药液附着最及渗入量,但不会使叶鞘、茎、穗中的积累量增多。 通过根系吸收的~(125)I-增产灵大部分留在根内,少部分向上运转,以叶鞘积累量较多,茎部次之,叶片及穗部运入量很少。 在水稻生育后期喷施增产灵,糙米中的标记物残留量在2ppm以下。 在显微放射自显影中,~(14)C-增产灵的轨迹首先在茎的维管束及其周围细胞中密集出现,其后分散在茎叶的薄壁组织中。标记物较多地积聚于叶、茎中,推论增产灵有调节韧皮部的运输和动员贮藏物向代谢中心运输的作用。
~ (14) C-Zenglingling was prepared with ~ (14) sodium C-chloroacetate; ~ 125 I-zoltralin was prepared with Na ~ (125) I. At the booting stage or in the early stage of lake lakes, foliar coating, spraying or pouring the root method is adopted to separately introduce the markers into the rice body. Three hours after application of ~ (125) I-splenicin (1000 ppm, 0.1 ml) to the leaf surface of the flag, the marker reached 75.2% of the attached amount in the leaf; a portion of the marker was transferred from the leaf to the leaf sheath, Spike and other leaves, of which the accumulation of leaf sheath more. The foliar application of ~ (125) I-Zenshenling (100 ppm) resulted in a 26.5% increase in the amount of infiltration within 1 hour, with an increase of absorption over time. The accumulation of the marker in the leaves was the highest, followed by the leaf sheaths, and the stems and spikes were few. Adding 0.1% soap solution increased the attachment and infiltration rate of the leaf surface, but did not increase the accumulation amount in leaf sheaths, stems and spikes increase. Most of the ~ (125) I-naringin absorbed by the roots remained in the roots, with a small part upwards, with more accumulated leaf sheaths, followed by the stems and less influx of leaves and ears. In the late rice growth by spraying Ling, brown rice residue in the marker 2ppm or less. In microscopic autoradiography, the trajectory of ~ (14) C-zenicillin first appeared densely in the vascular bundles and surrounding cells of the stem, which were then dispersed in the parenchyma of stems and leaves. More markers accumulate in leaves and stems, suggesting that ZSP can regulate the transport of phloem and mobilize the storage of the role of transport to the metabolic center.