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背景与目的:电磁脉冲(electromagneticpulse,EMP)辐射已用于饮食行业的灭菌,且较2450MHz连续微波辐射消毒更加有效。本研究探讨高能电磁脉冲对肺癌细胞GLC-82凋亡的影响,以发现治疗肿瘤的新手段。方法:以场强为6×104V/m的EMP2min内辐照5次,然后采用细胞计数、MTT、流式细胞术及SP免疫组化法检测bcl-2和p53蛋白的表达,并对表达强度用CMIAS-Ⅱ图像分析仪在放大400倍条件下进行分析,通过上述方法观察EMP对肺癌细胞GLC-82的损伤作用,所有数据经SPSS8.0软件进行分析。结果:EMP可明显抑制肺癌细胞GLC-82的增殖与活力。照射后细胞的MTT光吸收值(A570)与对照组相比,在辐照后0h,1h和6h明显降低。流式细胞术证明,GLC-82细胞在辐照后6h发生明显的凋亡,凋亡率达13.38%。免疫组化的图像分析表明,照射后GLC-82细胞有不同程度的bcl-2蛋白表达的下调及p53蛋白表达的上调。结论:EMP可诱导肺癌细胞GLC-82的凋亡。bcl-2及p53蛋白参与了GLC-82细胞的凋亡过程。
Background and Objective: Electromagnetic pulse (EMP) radiation has been used in the sterilization of the diet industry and is more effective than 2450 MHz continuous microwave radiation disinfection. This study was to investigate the effect of high-energy electromagnetic pulse on apoptosis of lung cancer cell line GLC-82 in order to find a new way to treat tumor. Methods: The cells were irradiated with EMP2min at a field intensity of 6 × 104V / m for 5 times. The expressions of bcl-2 and p53 proteins were detected by MTT assay, flow cytometry and SP immunohistochemistry. The CMIAS-Ⅱ image analyzer was used to analyze the effect of EMP on the GLC-82 lung cancer cells. The data were analyzed by SPSS8.0 software. Results: EMP significantly inhibited the proliferation and viability of GLC-82 cells. The MTT light absorbance (A570) of irradiated cells was significantly lower at 0h, 1h and 6h after irradiation than that of the control group. Flow cytometry demonstrated that GLC-82 cells showed obvious apoptosis at 6h after irradiation, with a apoptotic rate of 13.38%. Immunohistochemical image analysis showed that after irradiation GLC-82 cells have different degrees of bcl-2 protein expression downregulation and p53 protein expression. Conclusion: EMP can induce the apoptosis of GLC-82 lung cancer cells. Bcl-2 and p53 proteins are involved in the apoptosis of GLC-82 cells.