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目的 了解白细胞介素 6 (IL 6 )、肿瘤坏死因子 α(TNF α)在骨质疏松症患者腰椎骨中的细胞定位、表达强度及其与骨密度 (BMD)的关系。 方法 用免疫组织化学方法 ,分别以IL 6、TNF α单克隆抗体对 2 2例老年骨质疏松症患者腰椎椎板近关节突部松质骨组织中IL 6、TNF α的表达水平进行检测 ,并取 2 0例骨密度正常者相同部位骨作对照。 结果 实验组中IL 6、TNF α表达呈强阳性 ,主要表达于成骨细胞和骨髓基质细胞 ,每高倍视野阳性细胞数分别为 (10 7 9± 42 6 )个、(99 0±31 8)个 ;而对照组上述细胞表达呈弱阳性 ,每高倍视野阳性细胞数分别为 (31 4± 2 0 5 )个、(2 8 5±17 1)个 ,两组比较差异有显著性 (P <0 0 1)。试验组IL 6、TNF α阳性细胞数随BMD减低而增强 (相关系数分别为r1=- 0 91,P <0 0 1;r2 =- 0 84,P <0 0 1)。两组骨细胞IL 6、TNF α染色均为阴性。 结论 IL 6、TNF α作为调节骨代谢的细胞因子在骨质疏松症的发病机制中起一定作用
Objective To investigate the cell localization and expression of interleukin 6 (IL 6) and tumor necrosis factor α (TNF α) in the lumbar vertebrae of patients with osteoporosis and its relationship with bone mineral density (BMD). Methods Immunohistochemical method was used to detect IL 6 and TNFα expression in cancellous bone tissue of lumbar intervertebral facet joint in 22 elderly patients with osteoporosis by IL 6 and TNFα monoclonal antibodies respectively. Twenty patients with normal BMD were selected as controls. Results The expression of IL-6 and TNF-α in the experimental group was strongly positive and mainly expressed in osteoblasts and bone marrow stromal cells. The number of positive cells per high power field was (10 7 9 ± 42 6), (99 0 ± 31 8) (31 4 ± 2 0 5), (2 8 5 ± 17 1), respectively. The difference between the two groups was significant (P < 0 0 1). The number of IL-6 and TNF-α positive cells in experimental group increased with the decrease of BMD (r1 = -091, P <0.01; r2 = -084, P <0.01). Two groups of bone cells IL 6, TNF α staining were negative. Conclusion IL 6 and TNF α play a role in the pathogenesis of osteoporosis as cytokines that regulate bone metabolism