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目的： 为增强猪囊尾蚴抗原ｃ Ｃ１ 的免疫保护作用而构建一表达猪白细胞介素４（ Ｉ Ｌ４）与 ｃ Ｃ１ 抗原融合蛋白的 Ｄ Ｎ Ａ 疫苗载体。方法： 通过聚合酶链反应（ Ｐ Ｃ Ｒ），分别扩增猪 Ｉ Ｌ４ｃ Ｄ Ｎ Ａ 和ｃ Ｃ１ｃ Ｄ Ｎ Ａ 片段并进行融合，获得的嵌合基因 Ｉ Ｌ４ｃ Ｃ１ 中含有１０ 个氨基酸的中间接头序列，同时对其５’ Ａ Ｕ Ｇ 侧翼序列进行翻译优化突变。结果：经酶切鉴定，证实有一１．５ ｋｂ 的片段插入载体，其 Ｄ Ｎ Ａ 序列分析结果与文献报道和实验设计完全一致。结论：表达猪 Ｉ Ｌ４ 与猪囊尾蚴抗原ｃ Ｃ１ 融合蛋白的 Ｄ Ｎ Ａ 疫苗载体构建成功。
OBJECTIVE: To construct a D N A vaccine vector expressing porcine interleukin-4 (IL-4) and c C1 antigen fusion protein in order to enhance the immunoprotection of cysticercus cellulosae c C1. METHODS: The porcine I L4c D N A and c C1c D N A fragments were amplified respectively by polymerase chain reaction (PCR) and fused. The chimeric gene I L4c C1 contained 10 amino acids Of the middle linker sequence, while its 5 ’A U G flanking sequence for translational optimization mutations. Results: After identification by restriction enzyme digestion, a 1.5 kb fragment was inserted into the vector. The results of D N A sequence analysis were completely consistent with those reported in the literature and the experimental design. Conclusion: The D N A vaccine vector expressing pig I L4 and cysticercus cellulosae c C1 fusion protein was successfully constructed.