论文部分内容阅读
本文目的是利用扫描电子显微镜具有景深大,产生三维图象强等特点,对骨的正常形态结构变化做了较系统的观察,探讨了骨的各种细胞的超微结构,为进一步研究提供实验依据。 1 材料与方法动物,取成年正常健康家兔的胫、尺和颌骨。方法:①取材固定,用利锯将新鲜鱼骨,在不同部位,不同解剖层次锯成0.5～1.0cm骨片。生理盐水充分冲洗后,立即投入2％戊二醛固定液内。②导电染色,a、将固定的骨片用双蒸馏水充分冲洗后,放入导电
The purpose of this paper is to make a systematic observation of the normal morphological changes of bone using the characteristics of large depth of field and strong three-dimensional image generated by scanning electron microscope, discuss the ultrastructure of various cells in bone and provide experiment for further research in accordance with. 1 Materials and Methods Animals, to take adult normal tibia, ruler and jaw rabbit. Methods: ①Collected and fixed, with fresh sawdust fresh fish bone, in different parts, different anatomical levels of sawing 0.5 ~ 1.0cm bone chips. Physiological saline fully rinsed, immediately put 2% glutaraldehyde fixed solution. ② conductive staining, a, fixed bones with double distilled water thoroughly washed, into the conductive