用4～30%聚丙烯酰胺梯度凝胶盘状电泳测定人血清γ-谷氨酰转肽酶同工酶。此同工酶的Ⅰ′、Ⅱ、Ⅲ′带为原发性肝癌特异性区带,占74.8%(110/147),其中 AFP 阴性肝癌出现特异性区带者占44/63。其他病160例中只有3例出现特异性区带,孕妇、新生儿及正常人对照组不出现,假阳性率为1.2%(3/255).结果表明γ-谷氨酰转肽酶同工酶是一种诊断原发性肝癌的特异性肿瘤标记。 The human serum [gamma]-glutamyl transpeptidase isoenzyme was measured using a 4 to 30% polyacrylamide gradient gel electrophoresis. The I′, II, and III′ bands of this isoenzyme were the specific regions of primary liver cancer, which accounted for 74.8% (110/147), of which 44% were specific bands of AFP-negative liver cancer. Of the 160 patients with other diseases, only 3 showed specific bands. Pregnant women, neonates and normal controls did not appear. The false positive rate was 1.2% (3/255). The results showed that γ-glutamyl transpeptidase is the same. The enzyme is a specific tumor marker for the diagnosis of primary liver cancer.