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目的探讨金黄色葡萄球菌对系统性红斑狼疮(SLE)中性粒细胞胞外诱捕网(NETs)产生的影响及意义。方法收集SLE稳定者22例、活动者20例和健康对照18例,分离中性粒细胞并加金黄色葡萄球菌共培养,检测金黄色葡萄球菌刺激后NETs的主要成分,DNA试剂盒提取测定游离DNA(f DNA)及酶联免疫吸附法测定(ELISA)上清LL-37,并分析其与SLE临床及实验指标的关系。结果 1)SLE稳定组、活动组、健康对照组3组金黄色葡萄球菌刺激后f DNA量的比较差异有统计学意义,SLE活动组、稳定组均高于健康对照组(P<0.05),SLE活动组与稳定组无显著性差异(P>0.05);LL-37比较无统计学意义(P>0.05)。2)未经金黄色葡萄球菌刺激f DNA量3组间差异有统计学意义,SLE稳定组高于活动组和健康对照组(P分别是0.008、0.027),SLE活动组和健康对照组差异无统计学意义(P>0.05);LL-37比较无统计学意义(P>0.05)。3)3组体外金葡色葡萄球菌刺激后f DNA及LL-37量均较刺激前显著增加,差异均有统计学意义(f DNA P分别是<0.000 1、<0.000 1、0.001,LL-37 P均为<0.000 1);刺激前后f DNA的差值SLE活动组显著高于稳定组和健康对照组(P<0.05),SLE稳定组、健康对照组差异无统计学意义(P>0.05);LL-37差值组间比较均无统计学意义(P>0.05)。结论金黄色葡萄球菌刺激后,SLE患者NETs产生/释放量显著增加,SLE活动者最显著,金黄色葡萄球菌对SLE疾病活动可能有影响。
Objective To investigate the effect of Staphylococcus aureus on the production of neutrophil extracellular network (NETs) in patients with systemic lupus erythematosus (SLE). Methods Twenty-two patients with stable SLE, 20 active and 18 healthy controls were enrolled in this study. Neutrophils were isolated and staphylococcus aureus were co-cultured to detect the main components of NETs after S.aureus stimulation. The DNA kit was extracted and assayed for free LL-37 was detected by DNA (f DNA) and enzyme-linked immunosorbent assay (ELISA), and its relationship with clinical and laboratory parameters of SLE was analyzed. Results 1) There was significant difference in the amount of f DNA after stimulated by Staphylococcus aureus among the SLE stable group, active group and healthy control group. SLE active group and stable group were higher than healthy control group (P <0.05) SLE activity group and stable group no significant difference (P> 0.05); LL-37 was not statistically significant (P> 0.05). 2) There was a significant difference in the amount of DNA without Staphylococcus aureus between the three groups (P = 0.008,0.027, P <0.05). There was no difference between the SLE group and the healthy control group Statistical significance (P> 0.05); LL-37 was not statistically significant (P> 0.05). 3) The amounts of f DNA and LL-37 in three groups after stimulated by Staphylococcus aureus in vitro were significantly increased compared with those before stimulation (f DNA P were <0.0001, <0.0001.001, LL- (P <0.05). There was no significant difference between SLE stable group and healthy control group (P> 0.05) ); There was no significant difference between LL-37 difference groups (P> 0.05). Conclusions After S. aureus stimulation, NETs production / release increased significantly in patients with SLE, the most significant SLE activity, Staphylococcus aureus may affect SLE disease activity.