论文部分内容阅读
建立了高效毛细管电泳法拆分并测定尼群地平片和尼莫地平片中的光学异构体。采用未涂层弹性融硅石英毛细管柱,以40 mmol/L磷酸二氢钠溶液(以磷酸调至pH 2.96)-10 mmol/L磺丁基醚-β-环糊精为运行缓冲液,分离电压-15 kV,检测波长237 nm。尼群地平和尼莫地平对映体得到基线分离,分离度为3.43和2.08。考察了环糊精的浓度、缓冲液浓度和pH、分离电压等的影响,并运用计算机分子模拟技术对环糊精与对映体间的拆分机制进行探讨。
A high performance capillary electrophoresis method was established for the separation and determination of optical isomers in nitrendipine and nimodipine. Uncoated flexible fused silica capillary column was used with 40 mmol / L sodium dihydrogen phosphate solution (adjusted to pH 2.96 with phosphoric acid) -10 mmol / L sulfobutylether-β-cyclodextrin as running buffer, separated Voltage -15 kV, detection wavelength of 237 nm. The enantiomers of nitrendipine and nimodipine were baseline-resolved with resolution of 3.43 and 2.08. The effects of cyclodextrin concentration, buffer concentration and pH, separation voltage and so on were investigated. The resolution mechanism of cyclodextrin and enantiomer was discussed by computer molecular simulation.