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目的研究亲代大鼠转Bt基因水稻(TT51大米)暴露对其子代部分早期生理和神经发育指标的影响。方法亲代大鼠随机分为3组,每组雄性15只,雌性30只,分别给予按60%比例掺入市售大米、明恢63大米和TT51大米的饲料,连续喂养70 d后同组雄雌大鼠交配并产生子代,孕期和哺乳期各组母鼠继续给予相应受试大米的饲料。亲代大鼠每周称量和记录体重及食物消耗量,同时观察动物生长发育状况,观察和记录亲代雌鼠生殖指标。仔鼠出生后记录0、4、7、14及21 d体重,并观察仔鼠部分生理指标发育情况,同时进行听觉惊愕等神经发育指标的测定。断乳后,对各试验组部分仔鼠取脑等主要脏器进行病理检查。结果 TT51大米组与明恢63大米组和市售大米组比较,亲代大鼠一般毒性及生殖指标间差异均无统计学意义;子代部分生理发育指标和神经发育指标差异均无统计学意义;各试验组仔鼠脏器病理检查均未见有意义的变化。结论未见亲代大鼠转Bt基因水稻暴露对其子代早期部分生理和神经发育指标有不良影响。
Objective To study the effect of parental transgenic Bt rice (TT51 rice) exposure on some early physiological and neurodevelopmental indicators of offspring. Methods The offspring rats were randomly divided into three groups, 15 male and 30 female. The rats were fed with 60% of commercially available rice, Minghui 63 rice and TT51 rice respectively. After continuous feeding for 70 days, Female rats mating and generation of offspring, pregnancy and lactation in each group of mother rats continue to be given the corresponding test rice feed. The parental rats weighed and recorded the body weight and food consumption weekly, while observing the growth and development of animals, observing and recording reproductive indicators of female domestic mice. The pups were born after 0, 4, 7, 14 and 21 d weight recorded and observed some of the physiological indicators of offspring development, at the same time auditory amblyopia and other neurodevelopmental indicators were measured. After weaning, we took pathological examination of some of the major organs such as brain in each experimental group. Results There were no significant differences in the general toxicity and reproductive indicators between the TT51 rice group and the Minghui 63 rice group and the commercially available rice group. There were no significant differences in some physiological and neurological development indexes among the offspring. There was no significant change in the pathological examination of the organs of pups in each experimental group. Conclusion There is no adverse effect on the early physiological and neurodevelopmental indicators of offspring in the offspring of transgenic Bt rice without parental exposure.