By using cp SSR( chloroplast microsatellites) markers,the genetic relationship among Boehmeria varieties was analyzed. Five pairs of cp SSR primers with excellent amplification result,clear amplification band and good repeatability were screened from 22 pairs of cp SSR primers,and the polymorphism rate was22. 73%. By using these five pairs of cp SSR primers,16 polymorphic loci were amplified from eight experimental materials,with an average of 3. 2 loci. According to the results of cluster analysis,these experimental materials were divided into three categories. The clustering results were consistent with the classification results based on chloroplast gene sequences. The pairs using cp SSR (chloroplast microsatellites) markers, the genetic relationship among Boehmeria varieties were analyzed. Five pairs of cp SSR primers with excellent amplification result, clear amplification band and good repeatability were screened from 22 pairs of cp SSR primers, and the polymorphism rate was22. 73%. By using these five pairs of cp SSR primers, 16 polymorphic loci were amplified from eight experimental materials, with an average of 3.2 loci. According to the results of cluster analysis, these experimental materials were divided into three categories The clustering results were consistent with the classification results based on chloroplast gene sequences.