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目的:调查miR-122靶基因白细胞介素1A(IL-1A)3′翻译区(3′UTR)插入(ins)/缺失(del)多态性(rs3783553)与脑胶质瘤的关联。方法:采用聚合酶链反应检测161例脑胶质瘤患者和206名正常对照组人群rs3783553多态性。结果:对照组中del/del、ins/del和ins/ins基因型频率分别为37.9%、48.1%和14.1%;脑胶质瘤组中各基因型频率分别为47.8%、44.7%和7.5%。与del/del基因型相比,ins/ins基因型显著降低了脑胶质瘤的发病风险,OR=0.42,95%CI:0.20～0.88,χ2=5.45,P=0.02。对照组中del和ins等位基因频率分别为61.9%和38.1%;脑胶质瘤组中两等位基因频率分别为70.2%和29.8%。与del等位基因相比,ins等位基因显著降低了脑胶质瘤的发病风险,OR=0.69,95%CI:0.51～0.94,χ2=5.50,P=0.019。根据脑胶质瘤病理分级进行分层分析,ins和del等位基因在Ⅰ～Ⅱ级中的频率分别为69.5%和30.5%,在Ⅲ～Ⅳ级中的频率分别为71.2%和28.8%,两者相比差异无统计学意义,P>0.05。结论:miR-122靶基因IL-1A3′UTR区插入/缺失多态性(rs3783553)与脑胶质瘤发病相关。
OBJECTIVE: To investigate the association of miR-122 target 3’UTR insertion / deletion polymorphism (rs3783553) with glioma in IL-1A target gene. Methods: Polymorphisms of rs3783553 in 161 patients with glioma and 206 normal controls were detected by polymerase chain reaction. Results: The frequencies of del / del, ins / del and ins / ins genotypes in the control group were 37.9%, 48.1% and 14.1%, respectively. The frequencies of genotypes in the glioma group were 47.8%, 44.7% and 7.5% . Compared with the del / del genotype, the ins / ins genotype significantly reduced the risk of glioma, OR = 0.42, 95% CI: 0.20-0.88, χ2 = 5.45, P = 0.02. The frequencies of the del and ins alleles in the control group were 61.9% and 38.1%, respectively. The frequencies of the two alleles in the glioma group were 70.2% and 29.8%, respectively. Compared with the del allele, the ins allele significantly reduced the risk of gliomas, OR = 0.69, 95% CI: 0.51-0.94, χ2 = 5.50, P = 0.019. According to the histological grade of glioma, the frequencies of ins and del alleles were 69.5% and 30.5% in grade Ⅰ ~ Ⅱ and 71.2% and 28.8% in grade Ⅲ ~ Ⅳ, respectively. There was no significant difference between the two groups (P> 0.05). Conclusion: The insertion / deletion polymorphism of IL-1A3’UTR region of miR-122 target gene (rs3783553) is associated with the pathogenesis of glioma.