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目的:建立补益地黄丸中β-蜕皮甾酮含量分析的方法。方法:采用高效液相色谱(HPLC)法,FORTIS-C18柱(250 mm×4.6 mm,5μm),以乙腈-0.1%甲酸溶液(16∶84)为流动相,流速1.0 m L/min,柱温30℃,检测波长250 nm。结果:β-蜕皮甾酮在4.24~84.80μg/m L浓度范围内与峰面积有良好线性关系(r=0.9999),平均加样回收率为97.78%,RSD为1.77%。结论:该方法灵敏度高、准确、重复性好、专属性强,可作为控制补益地黄丸质量的重要方法。
Objective: To establish a method for the determination of β-ecdysterone in Buyi Dihuang Pill. Methods: High performance liquid chromatography (HPLC) was performed on a FORTIS-C18 column (250 mm × 4.6 mm, 5 μm) using acetonitrile-0.1% formic acid solution (16:84) Temperature 30 ℃, detection wavelength 250 nm. Results: β-ecdysterone showed a good linear relationship with the peak area in the range of 4.24-84.80 μg / mL (r = 0.9999), with an average recovery of 97.78% and a RSD of 1.77%. Conclusion: The method is sensitive, accurate, reproducible and specific, and can be used as an important method to control the quality of Rehmannia glutinous rice.