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目的以川续断的干燥根为原料,建立高速逆流色谱法(HSCCC)分离纯化川续断中续断皂苷Ⅵ的方法。方法川续断醇提物先经过AB-8大孔树脂富集目标物质。然后,以乙酸乙酯-正丁醇-水(3∶1∶4,v/v)为溶剂体系,轻相为固定相,重相为流动相,主机转速800 r/min,流速3.0 ml/min,ELSD检测,利用半制备型HSCCC分离纯化续断皂苷Ⅵ。结果经过大孔吸附树脂富集-高速逆流色谱法分离后,从200 mg川续断提取物中一次性得到续断皂苷Ⅵ75 mg,经HPLC检测其纯度为98.5%。结论本方法快捷简便,重复性好,为大批量制备生产川续断中续断皂苷Ⅵ提供了参考。
AIM: To establish a method for the separation and purification of Dipsacus saponins Ⅵ by high speed countercurrent chromatography (HSCCC) using the dried roots of Dipsacus as the starting material. Methods The alcohol extracts of S. argenteus were first enriched with AB-8 macroporous resin. Then, the mobile phase consisted of light phase as stationary phase and heavy phase as mobile phase with ethyl acetate-n-butanol-water (3:1:4, v / v) min, ELSD detection, the semi-preparative HSCCC isolation and purification continued saponin Ⅵ. Results After the macroporous resin was separated by high performance countercurrent chromatography, the yield of severed saponin Ⅵ75 mg was obtained from 200 mg Chuanxuan decoction once, and its purity was 98.5% by HPLC. Conclusion The method is rapid, simple and reproducible, which provides a reference for the preparation of Daphniandifolia chuanxiong Duan.