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为了在一些还没有孢子收集器的社队,尽快地普及蘑菇制种技术,本人选用三种不同成熟度的种菇,直接用无菌接种环在菌褶间刮取孢子分离培养,都获得了无杂菌的母种,成功率较高。菌膜破裂60～90%的种菇,菌丝长势较快,其次是菌膜初破的种菇,最慢的是菌膜全部破裂的种菇。杂菌感染率也高一些,但成功率仍达33%左右。有关三种种菇的孢子成熟度,生活力有待进一步研究。
In order to popularize the mushroom seed production technology as soon as possible in some community teams that do not yet have spore collectors, I chose three kinds of mushrooms with different maturation degrees to directly separate and cultivate the spores through the sterile loop, No bacteria mother species, a higher success rate. Mycotic membrane rupture of 60 to 90% of the mushrooms, mycelium grows fast, followed by the initial bacterial membrane mushrooms, the slowest of all the bacteria membrane rupture of mushrooms. Bacteriosis infection rate is higher, but the success rate is still up to 33%. The three kinds of mushroom spore maturity, vitality to be further studied.