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近期流行病学研究已发现胃幽门螺杆菌(HP)是胃癌发生的重要始发因素。本文应用敏感特异的聚合酶链反应(PCR)方法检测胃粘膜组织HP,分析其与C—Ha—ras第12位密码子点突变,ras基因产物P~(21)蛋白表达及DNA倍体的关系。发现C—Ha—ras点突变率于HP阳性组高于阴性组,并且HP阳性组较HP阴性组出现rasP~(21)表达增强,说明HP感染与C—Ha—ras基因活化有关,HP感染后DNA含量及S％期细胞明显增高,提示核苷酸代谢旺盛,DNA损伤及非整倍体的危险性也增高。本研究为HP致胃癌的可能机制从分子水平及细胞代谢水平作了初步研究。
Recent epidemiological studies have found that gastric Helicobacter pylori (HP) is an important origin of gastric cancer. In this study, a sensitive and specific polymerase chain reaction (PCR) method was used to detect HP in gastric mucosa. The mutations in C-Ha-ras codon 12 and the expression of ras gene P21 protein and DNA ploidy were analyzed. relationship. The C-Ha-ras point mutation rate was higher in the HP positive group than in the negative group, and the rasP~(21) expression was increased in the HP positive group compared to the HP negative group, indicating that the HP infection is related to the activation of the C-Ha-ras gene and the HP infection. After the DNA content and S% phase cells were significantly increased, suggesting that nucleotide metabolism is strong, DNA damage and the risk of aneuploidy is also increased. This study was a preliminary study of the possible mechanism of HP induced gastric cancer from the molecular level and the level of cellular metabolism.