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Cordyceps is a famous traditional Chinese medicine(TCM) that has been used in China for hundreds of years.In the present study a multi-column liquid chromatography(MC-LC) system was developed for the qualitative analysis of macromolecules and micromolecules in Cordyceps.The MC-LC system includes a size exclusion pre-column,a size exclusion column(SEC) and a reversed phase column(RP) which were controlled by column-switching valves.The sample was separated by the size exclusion pre-column into two fractions(macromolecules and micromolecules).These fractions were further separated on SEC and RP columns,respectively.A diode array detector(DAD) and a mass spectrometer(MS) were used to detect the components.This MC-LC method was utilized for analysis of Cordyceps samples.Two macromolecular peaks and 15 micromolecular peaks were found in Cordyceps,and 11 of the micromolecular peaks were identified as adenosine-5’-monophosphate(AMP),phenylalanine,uridine,hypoxanthine,inosine,guanine,guanosine,deoxyadenosine-5’-monophosphate(dAMP),adenosine,adenine and cordycepin(or its isomer).This method is useful for quality control of Cordyceps.
Cordyceps is a famous traditional Chinese medicine (TCM) that has been used in China for hundreds of years. The present study a multi-column liquid chromatography (MC-LC) system was developed for the qualitative analysis of macromolecules and micromolecules in Cordyceps. The MC-LC system includes a size exclusion column (SEC) and a size exclusion column (SEC) and a size exclusion column fractions (macromolecules and micromolecules). These fractions were further separated on SEC and RP columns, respectively. A diode array detector (DAD) and a mass spectrometer (MS) were used to detect the components. This MC- LC method was utilized for analysis of Cordyceps samples. Two macromolecular peaks and 15 micromolecular peaks were found in Cordyceps, and 11 of the micromolecular peaks identified as adenosine-5’-monophosphate (AMP), phenylalanine, uridine, hypoxanthine, inosine, guanine, guanosine , deoxyadenosine-5’-monophosphate (dAMP), adenosine, adenine and cordycepin (or its isomer). This method is useful for quality control of Cordyceps.