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目的研究蛋白酶激活受体-1(PAR-1)与肺癌侵袭、转移的关系。方法应用SP法对80例手术切除的肺癌组织标本进行PAR-1免疫组织化学染色;应用形态计量学分析方法结合临床病理资料分析PAR-1表达与肺癌转移相关指标的关系。结果80例肺癌组织中PAR-1阳性表达数为59例(73.8%)。有转移组的56例中PAR-1阳表达数为48例(85.7%),无转移组的24例中PAR-1阳性表达数为11例(45.8%),二者差异有显著性意义(p<0.01)。图像分析结果表明:有转移病例(7.84±6.11)和无转移病例(4.48±2.59)组间积分光密度的差异则有显著意义(p<0.05),肿瘤组织(6.505±3.086)与肺组织(2.91±1.97)及原发灶(6.505±3.086)与转移灶(10.827±2.20)各组之间积分光密度的差异度均有非常显著意义(p<0.01)。结论PAR-1可能在肺癌进展的过程中发挥着重要作用;PAR-1过度表达可能与肺癌的恶性表型及侵袭和转移表型有关。
Objective To study the relationship between the expression of protease-activated receptor-1 (PAR-1) and the invasion and metastasis of lung cancer. Methods PAR-1 immunohistochemical staining was performed on 80 surgically resected lung cancer specimens by SP method. The relationship between PAR-1 expression and lung metastasis-related indicators was analyzed by morphometric analysis and clinicopathological data. Results The positive expression of PAR-1 in 80 cases of lung cancer was 59 (73.8%). The positive expression of PAR-1 in 56 cases with metastasis was 48 cases (85.7%), and the positive expression of PAR-1 in 11 cases without metastasis was 11 cases (45.8%), the difference was significant p <0.01). The results of image analysis showed that the differences of integral optical density between metastatic cases (7.84 ± 6.11) and non-metastatic cases (4.48 ± 2.59) were significant (p <0.05), and those between tumor tissues (6.505 ± 3.086) and lung tissues 2.91 ± 1.97) and the difference of integral optical density between the primary (6.505 ± 3.086) and metastatic (10.827 ± 2.20) groups were significant (p <0.01). Conclusions PAR-1 may play an important role in the progression of lung cancer. PAR-1 overexpression may be related to malignant phenotype, invasion and metastasis phenotype of lung cancer.