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以人参根源愈伤组织为受体,分析了无土栽培条件下收集的人参原生根系分泌物的自毒作用。结果表明:人参根系分泌物可抑制愈伤组织的生长,且随处理浓度增加,抑制活性增强,质量浓度为200 mg/L时,生物量降低56.1%,与对照差异显著;可影响受体抗逆酶活性强弱及变化趋势,短时间或低浓度处理下,酶活性增加,其中超氧化物歧化酶活性增加幅度最大,其次为过氧化物酶和过氧化氢酶,而长时间或中高浓度处理后,酶活性均显著降低,同时3种酶原有的协调作用关系及平衡被破坏。人参原生根系分泌物具有化感自毒活性,对受体抗逆酶系统的显著影响是人参根系分泌物化感活性的重要表现。
The ginseng rooted callus was used as the receptor to analyze the autotoxicity of ginseng root exudates collected under soilless culture conditions. The results showed that: ginseng root exudates could inhibit the growth of callus, and with the increase of treatment concentration, the inhibitory activity was enhanced. When the concentration was 200 mg / L, the biomass decreased 56.1%, which was significantly different from the control; The enzyme activity and enzyme activity increased with the increase of short-term and low-concentration treatments. The activity of superoxide dismutase (SOD) increased the most, followed by peroxidase and catalase After treatment, the enzyme activity was significantly reduced, while the three kinds of enzymes have a coordinated role and the balance of the original is destroyed. The ginseng root exudates have the allelochemical autotoxicity, and the significant effect on the receptor anti-stress enzyme system is an important manifestation of the allelopathic activity of ginseng root exudates.