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为明确外源硒及脯氨酸(Pro)对菜用黄麻生长和生理特性的影响,本研究以新型菜用黄麻品种福农1号为材料,测定了在水培液中添加不同浓度的硒(0、0.2、0.5、1.0、2.5、5.0 mg/L)与脯氨酸(50μmol/L)对菜用黄麻苗期生长、叶绿素、渗透调节物质及抗氧化酶活性的影响。结果表明:(1)外源硒浓度≤0.5 mg/L时,能促进菜用黄麻的株高、根长、株重及根重,而外源硒浓度≥2.5 mg/L时则会对其生长产生抑制和毒害作用;浓度为0.2 mg/L的硒最有利于菜用黄麻福农1号苗期的生长发育;硒浓度较低时,外源脯氨酸具有促进菜用黄麻生长的作用,而硒浓度较高时,脯氨酸可缓解硒对菜用黄麻生长的抑制。(2)外源硒可促进菜用黄麻中叶绿素的合成,亦能促进游离脯氨酸、可溶性糖和可溶性蛋白等渗透调节物质的形成,并且随硒浓度增加呈现先升高后降低的变化趋势;硒浓度为0.5和1.0 mg/L时,可最大程度地促进菜用黄麻叶绿素和渗透调节物质的合成;外源脯氨酸亦具有促进菜用黄麻叶绿素和渗透调节物质合成的功能,并且对高浓度硒造成的毒害有一定的缓解作用。(3)中低浓度的硒(0.5和1.0 mg/L)明显促进菜用黄麻超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)等抗氧化酶的活性,而高浓度硒(5.0 mg/L)抑制菜用黄麻抗氧化酶的活性;随外源硒浓度增加,菜用黄麻抗氧化酶活性呈先升高后降低的趋势;外源脯氨酸能促进菜用黄麻抗氧化酶的活性,硒浓度为2.5和5.0 mg/L时,添加脯氨酸的处理POD、SOD和CAT活性高于未添加者,表明脯氨酸具有增强菜用黄麻抵抗力和自由基清除的能力,从而可以缓解高浓度硒的胁迫。以上结论对菜用黄麻这一新型蔬菜的生产具有重要的理论和实践意义。
In order to clarify the effects of exogenous selenium and proline (Pro) on the growth and physiological characteristics of jute for vegetables, a new vegetable jute variety, Fu-nong 1, was used to study the effects of different concentrations of selenium (0,0.2,0.5,1.0,2.5,5.0 mg / L) and proline (50μmol / L) on the growth, chlorophyll, osmotic adjustment substance and antioxidant enzyme activities of the germplasm in jute. The results showed that: (1) When the concentration of exogenous selenium was less than 0.5 mg / L, the plant height, root length, plant weight and root weight of jute could be promoted. When the concentration of exogenous selenium was ≥2.5 mg / L, Growth inhibition and poisoning effect; Selenium concentration of 0.2 mg / L is most conducive to the growth and development of the vegetable jute Fu-nong No. 1 seedling; low concentrations of selenium, exogenous proline has the effect of promoting the growth of jute , While the high concentration of selenium, proline can alleviate the inhibition of selenium on the growth of jute vegetables. (2) Exogenous selenium promoted the synthesis of chlorophyll in vegetable jute, and also promoted the formation of osmolytes such as free proline, soluble sugar and soluble protein, and increased with the increase of selenium concentration. At selenium concentrations of 0.5 and 1.0 mg / L, the synthesis of jute chlorophyll and osmotic adjustment substances in vegetables can be maximally promoted. Exogenous proline also has the function of promoting the synthesis of vegetable jute chlorophyll and osmoregulation substances, Poison caused by high concentrations of selenium have a certain degree of relief. (3) Low concentrations of selenium (0.5 and 1.0 mg / L) significantly promoted the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and glutathione Peroxidase (GSH-Px) and other antioxidant enzymes activity, while the high concentration of selenium (5.0 mg / L) inhibited the activity of jute antioxidant enzymes in vegetables. With the increase of exogenous selenium concentration, the activity of jute antioxidant enzyme The content of POD, SOD and CAT increased at the selenium concentration of 2.5 and 5.0 mg / L, respectively, when proline was added Not added, indicating that proline has increased vegetable jute resistance and free radical scavenging capacity, which can alleviate the high concentration of selenium stress. The above conclusion has important theoretical and practical significance for the production of vegetable jute, a new type of vegetable.