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目的提取中药甘草提取物(GL-1),研究其对人胃癌SGC-7901细胞的增殖抑制及凋亡诱导作用。方法将甘草粉末溶于水后取沉淀部分、经丙酮萃取、甲醇分离处理,得到甘草提取物GL-1。通过MTT比色法观察不同浓度的GL-1对SGC-7901细胞增殖抑制情况;AO(吖啶橙)/EB(溴化乙锭)双荧光染色法检测SGC-7901细胞凋亡的形态学变化;流式细胞仪测定各浓度组细胞凋亡率。结果 GL-1对SGC-7901细胞的生长增殖有抑制作用,在12、24、48 h作用于SGC-7901细胞的半数抑制浓度分别为24.18、20.03、15.60μg/m L(P<0.05);AO/EB染色结果表明12.5、25、50μg/m L剂量组SGC-7901细胞均有不同程度的凋亡作用;流式细胞仪检测结果表明,GL-1对SGC-7901细胞的诱导凋亡作用具有浓度依赖性。结论 GL-1具有抑制SGC-7901细胞生长增殖作用,并能诱导SGC-7901细胞凋亡。
Objective To extract Chinese medicinal licorice root extract (GL-1) and study its antiproliferative effects on human gastric cancer cell line SGC-7901. Method The glycyrrhiza powder was dissolved in water and the precipitated fraction was taken. The extract was extracted with acetone and separated by methanol to obtain licorice extract GL-1. The inhibitory effects of different concentrations of GL-1 on the proliferation of SGC-7901 cells were observed by MTT colorimetric assay. The morphological changes of SGC-7901 cells were detected by double staining of AO (acridine orange) / EB Flow cytometry was used to determine the apoptosis rate of each concentration group. Results GL-1 inhibited the proliferation of SGC-7901 cells. The half-inhibitory concentrations of GL-1 on SGC-7901 cells were 24.18,20.03,15.60μg / m L at 12,24,48 h (P <0.05), respectively. The results of AO / EB staining showed that SGC-7901 cells in 12.5, 25 and 50μg / ml dose groups all had different degree of apoptosis. The results of flow cytometry showed that GL-1 induced apoptosis in SGC-7901 cells Concentration-dependent. Conclusion GL-1 can inhibit the proliferation of SGC-7901 cells and induce the apoptosis of SGC-7901 cells.