论文部分内容阅读
鉴于鸡新城疫病毒(NDV)对人体无致病力、来源方便等优点,本文应用其强毒株NC_(48)CE_8和弱毒株HB_1介导融合体外培养的哺乳类细胞,包括:人体肝癌BEL-7402和BEL-7404系细胞、人宫颈癌Hela 细胞、人羊膜FL 细胞以及小鼠A9/28 DKS 系细胞。在本实验的最适条件下,NC_(48)CE_8株与HB_1株介导BDL-7404系细胞的融合率分别高达86.9%和60.7%。NDV 介导的细胞融合率与其稀释度及细胞的种系密切有关。借显微缩时电影术示范了BEL-7404系细胞在NC_(48)CE_8株与HB_1株介导下的融合过程。亚显微结构的分析,示明BEL-7404系细胞的表面,特别是在细胞紧密邻接的区域存在着大量的微绒毛。文内对此及融合所需的实验条件作了讨论。
In view of the virulence of Newcastle disease virus (NDV) on human body and its convenient source, the mammalian cells cultured in vitro with virulent strain NC_ (48) CE_8 and attenuated strain HB_1 were used in this study, including human hepatocellular carcinoma BEL-7402 And BEL-7404 lineage cells, human cervical cancer Hela cells, human amniotic FL cells, and mouse A9 / 28 DKS lineage cells. Under the optimal conditions of this experiment, the fusion rates of NC_ (48) CE_8 and HB_1 strains were up to 86.9% and 60.7%, respectively. NDV-mediated cell fusion rate is closely related to its dilution and cell germline. The microcosmic time-dependent cinematography demonstrated the fusion process of BEL-7404 cells under the NC_ (48) CE_8 strain and HB_1 strain. Sub-microstructural analysis revealed a significant amount of microvilli on the surface of BEL-7404 cells, especially in the immediate vicinity of the cells. The article discusses the experimental conditions required for this and fusion.