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毛细管电泳、变性色谱、免疫吸附、DNA测序方法等已广泛用于DNA碱基错配、氧化缺失、链断裂等变化的检测，但有时如进行药物筛选时，只需定性地检测DNA是否变化或变化程度。本文采用褶合光谱法定性地检测紫外线致DNA变化程度，将突变后DNA的褶合光谱与未变异前DNA自身标准比较，并以差谱值δ量化地表示突变程度。当褶合光谱δ高达11.48%时，才能从二阶导数光谱上发现差异，表明方法的灵敏度远远高于前者。加入DMSO后，溶液在254 nm照射时，δ升高，表现为DNA变异诱导剂；溶液在365 nm照射时，δ降低，表现为DNA变异保护剂。褶合光谱法快速、简便、灵敏、经济，可以作为检测DNA突变、筛选抗突变药物的一种新型方法。
Capillary electrophoresis, denaturation chromatography, immunosorbent, DNA sequencing methods have been widely used for DNA base mismatch, oxidative deletion, strand breaks and other changes in detection, but sometimes for drug screening, only qualitatively detect DNA changes or Degree of change. In this paper, convolution spectrometry was used to qualitatively detect the change of DNA induced by ultraviolet light. The convolutional DNA of the mutated DNA was compared with the standard of DNA before mutation, and the degree of mutation was quantitatively expressed by the difference spectrum δ. When the convolution spectrum δ is as high as 11.48%, the difference can be found from the second derivative spectrum, indicating that the sensitivity of the method is much higher than the former. After DMSO was added, the δ increased when the solution was irradiated at 254 nm, showing a DNA mutation inducer; when the solution was irradiated at 365 nm, the δ decreased, showing the DNA mutation protective agent. Convolution spectrometry is a fast, simple, sensitive and economical method that can be used as a new method to detect DNA mutations and screen anti-mutagenic drugs.