以玻碳电极为工作电极，硫酸钾为支持电解质，在合适的ｐＨ条件下，通过循环伏安法，首次观测到了马钱子碱的不可逆氧化峰。在浓度为２０×１０－４～４０×１０－３ｍｏｌ／Ｌ范围内其峰电流与浓度呈线性关系，检测限为１０×１０－５ｍｏｌ／Ｌ。在正常人体的尿样中，采用标准加入法进行回收实验，其平均回收率为１００８％。该法简便、快速，用５０×１０－４ｍｏｌ／Ｌ的马钱子碱溶液重复测定１０次，相对标准偏差为１８％。对其反应机理作了初步探讨。 Using glassy carbon electrode as working electrode and potassium sulfate as supporting electrolyte, the irreversible oxidation peak of brucine was firstly observed by cyclic voltammetry at the proper pH. The peak current was linear with the concentration in the range of 2 × 10-4 ~ 4 × 10-3 mol / L with the detection limit of 1.0 × 10-5 mol / L. In normal human urine samples, the standard addition method was used for recovery experiments, with an average recovery of 100.8%. The method is simple and rapid, with 10 × 10-4mol / L strychnine solution repeated determination of 10 times, the relative standard deviation of 18%. The reaction mechanism was preliminarily discussed.