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目的:分析本市贝类中副溶血性弧菌分离株和部分散发腹泻病例及食物中毒分离株的分子生物学特性,了解本市副溶血弧菌的流行规律。方法:对50株贝源分离株和18株临床分离株进行部分血清分型及tdh、trh、orf8和toxRS/new四种毒力基因检测,并对所有菌株进行RAPD分子分型研究。结果:50株贝源株分属于7个血清群,其中O:1群、O:10群、O:11群各占22%,O:4群占14%,O:2群占10%,O:5群占6%,O:3群占2%。tdh、orf8和toxRS/new这三种毒力基因检测结果均为阴性,只检出2株trh阳性株。18株人源分离株有13株属于O:3群,且均为O3:K6型,占72.2%,4株属于O:1群,1株属于O:4群。毒力基因检测中有14株tdh、orf8和toxRS/new三种毒力基因为阳性,1株trh阳性,其余3株四种毒力基因均为阴性,说明这18株中有14株(占77.78%)属于大流行株,其中13株为O3:K6型,1株为O1:KUT型;RAPD分型结果显示:50株贝类分离株电泳得到21种带型,主要型别为3型、12型和23型,占58%,其中菌株分布最集中的是23型,占38%。18株人源分离株分得5型、22型、23型共3种带型,分别占5.56%、5.56%和88.88%。结论:舟山市海产品中携带的副溶血性弧菌呈多样性分布,病人体内分离的副溶血性弧菌分布则相对比较集中;一群关系密切、tdh阳性、toxRS/new阳性、orf8阳性的O3:K6克隆群在舟山成优势流行。
OBJECTIVE: To analyze the molecular biological characteristics of Vibrio parahaemolyticus isolates and some diarrhea cases and food poisoning isolates in this city, and to understand the prevalence of Vibrio parahaemolyticus in this city. Methods: The partial serotyping and the tdh, trh, orf8 and toxRS / new virulence genes were detected in 50 Beiyuan isolates and 18 clinical isolates, and all the strains were studied by RAPD molecular typing. Results: 50 strains of Beiyuan belonged to 7 serogroups, of which O: 1, O: 10, O: 11 accounted for 22%, O: 4 accounted for 14%, O: 2 accounted for 10% O: 5 groups accounted for 6%, O: 3 groups accounted for 2%. tdh, orf8 and toxRS / new these three virulence gene test results were negative, only two strains were detected trh positive. Thirteen of the 18 human isolates belonged to the O: 3 group, all of which were O3: K6 type, accounting for 72.2%. Four strains belonged to O: 1 group and one strain belonged to O: 4 group. Among the virulence genes, 14 strains of tdh, orf8 and toxRS / new were positive for virulence genes, 1 for trh and 3 for the remaining 4 strains were negative, indicating that 14 of these 18 strains accounted for 77.78%) belonged to the pandemic strain, of which 13 strains were O3: K6 type and 1 strain was O1: KUT type. The results of RAPD typing showed that 21 strains of 50 strains of shellfish were electrophoresed and the major type was type 3 , Type 12 and type 23, accounting for 58%, of which 23 strains were the most concentrated, accounting for 38%. 18 human isolates were divided into 5 types, 22 types and 23 types, accounting for 5.56%, 5.56% and 88.88% respectively. Conclusion: The Vibrio parahaemolyticus carried by seafood in Zhoushan City showed a diversity distribution. The distribution of Vibrio parahaemolyticus in patients was relatively concentrated. A group of closely related, tdh positive, toxRS / new positive, orf8 positive O3 : K6 clonal group became predominantly popular in Zhoushan.