论文部分内容阅读
目的探讨9L/Fischer344和C6/Wistar两种大鼠脑胶质瘤模型的对比研究。方法采用立体定向技术,大鼠脑内接种定量的肿瘤细胞建立模型,比较两种模型大鼠脑内肿瘤生长情况;采用免疫组织化学方法,比较两种模型脑内肿瘤局部细胞免疫反应情况。结果(1)9L/Fischer344大鼠脑内有肿瘤持续生长,而C6/Wistar大鼠脑内早期可见肿瘤生长,但肿瘤局部有大量淋巴细胞浸润,肿瘤周边血管呈现“淋巴细胞血管套”现象,后期脑内肿瘤逐渐消退、消失,仅见残留轨迹和含褐色素沉着的吞噬细胞;(2)9L/Fischer344肿瘤局部可见CD68阳性细胞,未见CD4、CD8阳性细胞,而C6/Wistar肿瘤局部CD68阳性细胞数量明显较9L模型多(P<0.01),并可见CD4、CD8阳性的淋巴细胞。结论9L/Fischer344模型稳定性高,重复性好,肿瘤局部T淋巴细胞浸润极少,是一种较为理想的脑胶质瘤免疫治疗研究的动物模型;C6/Wistar模型脑内肿瘤不能持续生长,脑内肿瘤可自发性消退,肿瘤局部T淋巴细胞浸润明显,存在较强的细胞免疫反应,可能是肿瘤的消退的原因,该模型不适合用于胶质瘤治疗,尤其是免疫治疗的实验研究。
Objective To investigate the comparative study of glioma models between 9L / Fischer344 and C6 / Wistar rats. Methods Stereotactic technique was used to establish a model of rat brain by inoculation of quantitative tumor cells. The tumor growth of the two models were compared with each other. Immunohistochemistry was used to compare the local cellular immune response in the two models. Results (1) The tumors in 9L / Fischer344 rat brain continued to grow, whereas in C6 / Wistar rat brain, tumor growth was observed in the early stage. However, there were a large number of lymphocytic infiltration in the tumor and the phenomenon of “lymphocyte neoplasm” In the latter part of the brain tumor gradually subsided and disappeared, leaving only traces and brown pigmentation of phagocytic cells. (2) CD68 positive cells were found locally in 9L / Fischer344 tumor, no CD4 and CD8 positive cells were found, while CD68 positive in C6 / Wistar tumor The number of cells was significantly more than the 9L model (P <0.01), and CD4, CD8-positive lymphocytes were seen. Conclusion The 9L / Fischer344 model is an ideal animal model for glioma immunotherapy. It has high stability, good repeatability and minimal local infiltration of T lymphocytes. C6 / Wistar model can not sustain the growth of brain tumors, Brain tumors can spontaneously recede, local tumor T lymphocytes infiltration significantly, there is a strong cellular immune response may be the reason for tumor regression, the model is not suitable for the treatment of glioma, especially in the experimental study of immunotherapy .