论文部分内容阅读
目的:探讨补骨脂素(PSO)加长波紫外线(UVA)光化学疗法(PUVA)诱导人白血病细胞NB4、K562凋亡时对Fas、FasL表达的影响。方法:以NB4、K562细胞为研究对象,以细胞凋亡率、电镜下细胞超微结构改变以及细胞Fas、FasL在基因、蛋白水平的表达为检测指标,观察补骨脂中提取的PSO加波长为360 nm的UVA对人白血病细胞诱导凋亡的作用以及部分作用途径,并采用多因素方差分析法进行统计学处理。结果:PSO,UVA照射及PUVA均可诱导NB4、K562细胞发生凋亡,PUVA的作用显著强于前两者。电镜下观察经PUVA处理后的NB4、K562细胞超微结构,可见明显的凋亡形态学改变;PSO、UVA照射及PUVA均可上调NB4、K562细胞Fas在基因、蛋白水平的表达,下调FasL在基因、蛋白水平的表达,PUVA的作用显著强于前两者。结论:PUVA可诱导白血病细胞NB4、K562发生凋亡,其作用强于PSO及UVA照射,作用途径之一为上调细胞Fas基因表达水平,下调FasL基因表达水平。
Objective: To investigate the effect of psoriasis (PSO) plus long-wave ultraviolet (UVA) photochemical therapy (PUVA) on the expression of Fas and FasL in human leukemia cell line NB4 and K562. METHODS: The NB4 and K562 cells were studied. The apoptosis rate, the ultrastructure of cells under electron microscope, and the expression of Fas and FasL at the gene and protein level were used as detection indicators to observe the PSO wavelength extracted from psoralen. For 360 nm UVA induced apoptosis in human leukemia cells and part of the role of the way, and using a multivariate analysis of variance method for statistical analysis. RESULTS: PSO, UVA irradiation and PUVA all induced apoptosis in NB4 and K562 cells. The effect of PUVA was significantly stronger than that of the former two. The ultrastructure of NB4 and K562 cells treated with PUVA was observed under electron microscope, and obvious morphological changes of apoptosis were observed. PSO, UVA irradiation and PUVA up-regulated the expression of Fas gene and protein in NB4 and K562 cells, and down-regulated FasL expression. The expression of genes and protein levels, PUVA was significantly stronger than the first two. Conclusion: PUVA can induce apoptosis of NB4 and K562 leukemia cells, which is more potent than PSO and UVA irradiation. One of the pathways is up-regulation of Fas gene expression and down-regulation of FasL gene expression.