Glioma is a malignant brain tumor with a poor prognosis. Surgical resection is usually the first line of treatment. However, animal models of glioma do not include surgical resection and tumors are typically treated before they become advanced. This report demonstrates the feasibility of surgical resection of advanced gliomas in mice. The described technique establishes a murine model which could be used for the development of immunotherapy for advanced glioma after surgical resection. Use of surgical resection in murine models could increase the probability that therapies developed in mice will translate to human patients.