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目的：调查边境地区莱姆病和自然疫源地的状况。方法：对血清学、病原学、宿主、媒介、病例和地理景观方面进行调查。结果：用ＩＦＡ和ＥＬＩＳＡ法共检测人畜血清１ ５０１ 份，从１９９ 份人血清中检出抗体阳性１８ 份，阳性率为９ ．０５％ ，并发现莱姆病典型患者１５ 例；检查马血清２０ 份，阳性５ 份，阳性率为２５ ．００ ％ ；检查牛血清３９９ 份，阳性６９ 份，阳性率１７ ．２９ ％ ；检查羊血清８８３ 份，阳性１９９ 份，阳性率２２．５４ ％ 。调查地区为山间谷地，本次调查以森林、森林草原、草原和河漫滩草地生境为主。从四类生境采集蜱３０９０ 只和鼠类１２ 只。这些蜱共计８ 种，森林革蜱和银盾革蜱占绝大多数，中肠带菌率分别为１８ ．７５ ％ （６／３２） 和１３ ．３３ ％ （４／３０）。将蜱分为８６ 组，鼠类分为１２ 组分别接种培养于ＢＳＫ培养基，结果５ 个组培养出伯氏疏螺旋体（ Ｂｏｒｒｅｌｉａ ｂｕｒｇｄｏｒｆｅｒｉ） ，其中棕背 （ Ｃ．ｒｕｆｏｃａｎｕｓ） ２ 组，鼹形田鼠（ Ｅ．ｔａｌｐｉｎｕｓ）１ 组和森林革蜱（ Ｄ． ｓｉｌｖａｒｕｍ） ２ 组。结论：基于上述调查研究结果和流行病学资料分析，该地区为莱姆病自然疫源地。
Purpose: To investigate the status of Lyme disease and natural foci in the border areas. Methods: Serological, etiological, host, media, case, and geographic landscapes were investigated. RESULTS: A total of 1 501 serum samples were detected by IFA and ELISA. 18 antibody positive samples were obtained from 199 human serum samples. The positive rate was 9. 05%, and found that Lyme disease typical 15 patients; check serum 20, positive 5, the positive rate of 25. 00%; check bovine serum 399, positive 69, the positive rate of 17. 29%; check the sheep serum 883, positive 199, the positive rate of 22.54%. The survey area is mountain valleys. The survey mainly focused on forest, grassland, grassland and river floodplain grassland habitat. Totally 3090 ticks and 12 rodents were collected from four habitats. A total of eight of these ticks, the majority of forest brown ticks and silver shield mites, midgut carrier rates were 18. 75% (6/32) and 13. 33% (4/30). The ticks were divided into 86 groups, the mice were divided into 12 groups were inoculated and cultured in BSK medium, the results of five groups cultured Borrelia burgdorferi (Borrelia burgdorferi), which back group (C.rufocanus) 2, (Group E.talpinus) and group D. silvarum (group 2). Conclusion: Based on the above investigation and epidemiological analysis, the area is a natural foci of Lyme disease.